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Frozen Bacteria Repair Own Dna For Millennia


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August 27, 2007 Bacteria can survive in deep freeze for hundreds of thousands of years by staying just alive enough to keep their DNA in good repair, a new study says.


In earlier work, researchers had found ancient bacteria in permafrost and in deep ice cores from Antarctica.


These bacteria, despite being trapped for millennia, were able to be revived and grown in the lab. Some researchers had thought that bacteria would have to turn into dormant spores to survive for so long.


But if bacteria merely went dormant, metabolism would stop and various environmental factors would begin damaging their DNA.


Like an ancient scroll that's crumbling apart, the DNA becomes so damaged that it's indecipherable after about a hundred thousand years. Then the cells can't ever reproduce and the bacteria are effectively dead.


"Our results show that the best way to survive for a long time is to keep up metabolic activity," said Eske Willerslev, lead study author and a researcher at the University of Copenhagen in Denmark.


Doing this "allows for continuous DNA repair," Willerslev added.


The work suggests that if bacterial life existed on Mars or on Jupiter's moon Europa, it might still survive locked in icy soils.


The new study appears this week in the online advance edition of the Proceedings of the National Academy of Sciences.


Living, Just Barely


The new study examined DNA from bacteria found in permafrost from Siberia in Russia and Canada. The permafrost dated back to about a half-million years ago.



What the scientists found is that the bacteria appear to have kept up their metabolism. These barely living bacteria did not seem to be reproducing, but they were still taking in nutrients and giving off carbon dioxide, like humans do when they breathe.


The bacteria were using some of these resources to keep their DNA in good shape, the study authors said.


But the researchers found that bacteria couldn't keep chugging along like this forever.


"You see a large diversity [of bacteria] in the modern samples, and as you get older and older, the diversity declines," Willerslev said.


The amount of carbon dioxide the bacteria gave off also dropped with age.


The limit for life in the permafrost is somewhere around 600,000 years old, the researchers say.


In older permafrost, the team couldn't detect any carbon dioxide emissions or any large pieces of DNA indicative of living bacteria.


By about 750,000 years old, the bacteria trapped in the permafrost seemed to be completely dead.


Soil vs. Ice


Some scientists have claimed to be able to revive far older bacteria preserved in amber or salts, but Willerslev has doubts about these results.


"I've been extremely skeptical about these previous results," Willerslev said.


But in the much colder environments of Mars or Europa, life might be able to survive while frozen for much longer, Willerslev said.


At those lower temperatures, DNA damage would accumulate more slowly.


So the new results "could suggest that if you had similar life on Mars, it could exist for much longer," he said.


Brent Christner of Louisiana State University welcomes the new results, which he finds convincing.


Christner and others have been studying ancient ice from deep in the Antarctic ice sheet and have found live bacteria there that have been frozen in place for perhaps one to two million years.


These ancient bacteria seemed to be repairing themselves, but the team didn't have direct evidence showing how the microbes were surviving so long.


"This study confirms and corroborates everything we've been finding with ancient glacial ice," Christner said.


Still, Willerslev is cautious about making this connection.


Glacial ice, he said, "is a completely different environment from permafrost, which is basically frozen soil" and contains lots of nutrients.



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